Eleven of the 10.8m Enviro200 EV single-deckers are now operating on route 153, starting from Northumberland Park Garage in north London.
The depot has charging points designed and manufactured by BYD.
“We are delighted to make another significant step in the electrification of London's bus routes and to be in the vanguard of the transformation,” said Richard Harrington, Go-Ahead Group’s engineering director.
Frank Thorpe, UK manager for BYD and speaking on behalf of the ADL-BYD partnership, said: “The smooth switch on of our electric buses to operate another intensive London route is a testament to the strength of our overall offering – not just the proven and reliable buses themselves, but of the back-up and support of our partners in planning and installing the necessary equipment to make electric bus operation successful from day one.”